qScriber™ cDNA Synthesis Kit100 r of 20 µl

Applications:

- cDNA template generation for qPCR or PCR

- Unbiased, efficient cDNA synthesis

- Detection of low target amounts

- cDNA synthesis from complex templates

Benefits:

- Thermostable HighScriber™Reverse Transcriptase blended with Ribonuclease Inhibitor for efficient cDNA synthesis

- Optimized reaction mix with oligo (dT) and random primers for unbiased representation of mRNA ends

- cDNA synthesis from complex templates at up to 55°C

- High sensitivity detection from 1 pg of total RNA template

The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates.

The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends.

The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR.