innuTaq DNA-Polymerase

Specifications

Concentration
5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

PCR Buffer
10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
10× PCR Buffer with (NH₄)_2SO4; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH₄)_2SO4; 0.1 % Tween 20

Enzyme storage buffer
20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol

PS-Mg₂+ solution
MgCl₂ stock solution, 25 mM